A second site-specific restriction endonuclease from Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
A restriction endonuclease from Staphylococcus aureus.
A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded phi X174 DNA. It recognizes the sequence (see article) and cleaves as indicated by the arrows. Evidence is presented that this enzyme plays a role in the ...
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PCR multiplexing has proven to be challenging, and thus has provided limited means for pathogen genotyping. We developed a new approach for analysis of PCR amplicons based on restriction endonuclease digestion. The first stage of the restriction enzyme assay is hybridization of a target DNA to immobilized complementary oligonucleotide probes that carry a molecular marker, horseradish peroxidase...
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A second s i t e s p e c i f i c endonuclease with novel s p e c i f i c i t y has been pur i f i ed from Thermus thermophilus s t r a i n 111 and named T i T i l l l I I . The enzyme i s a c t i v e a t temperature up t o 80°C and r e q u i r e s Mg or Mn^ for endonucl ea se a c t i v i t y . TiTzll lII c leaves 4>X174RFDNA i n t o 11 fragments and lambda DNA i n t o more than 25 fragments. Fr...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1978
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/5.4.1153